3T6Z
Crystal Structure of Steccherinum ochraceum Laccase obtained by multi-crystals composite data collection technique (60% dose)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-02 |
| Detector | MARMOSAIC, 225 MM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.510, 140.040, 172.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 2.150 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.23200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3t6v |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.595 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.115 | 0.298 |
| Number of reflections | 88328 | |
| <I/σ(I)> | 7.51 | 3.22 |
| Completeness [%] | 90.3 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 296 | 1.9 M ammonium sulphate, 0.01 M zinc chloride, pH 9.0, vapor diffusion, temperature 296K |
