3T6U

Crystal Structure of Lysozyme in 40% sucrose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-08-23
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	77.614, 77.614, 37.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.526 - 2.213
R-factor	0.1917
Rwork	0.190
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sp3
RMSD bond length	0.015
RMSD bond angle	1.122
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.526	2.250
High resolution limit [Å]	2.210	2.210
Rmerge	0.045	0.186
Number of reflections	6134
<I/σ(I)>	25.7	4.8
Completeness [%]	91.0	68.5
Redundancy	2.7	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	277	40mM Sodium acetate, 150mM Sodium chloride, 40% Sucrose , pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K