3T6O

The Structure of an Anti-sigma-factor antagonist (STAS) domain protein from Planctomyces limnophilus.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97935, 0.97945
Spacegroup name	I 2 2 2
Unit cell lengths	73.038, 92.453, 142.753
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.17645
Rwork	0.174
R-free	0.22322
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.536
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.101	0.085	0.452
Number of reflections	27534
<I/σ(I)>	7.2
Completeness [%]	97.1	97.7	83.4
Redundancy	8.7	8.4	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.8M LiCl2, 0.1 Tris:HCl pH8.5, 32% PEG 4K, VAPOR DIFFUSION, SITTING DROP, temperature 289K