3T6L
Y54F mutant of core streptavidin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-01-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 57.273, 57.273, 171.715 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.628 - 1.300 |
R-factor | 0.1366 |
Rwork | 0.135 |
R-free | 0.16000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mm9 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.826 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.628 | 1.210 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.057 | 0.967 |
Number of reflections | 35679 | |
<I/σ(I)> | 21.8 | 2.3 |
Completeness [%] | 99.1 | 93.6 |
Redundancy | 14.7 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 2.5 M sodium chloride, 0.1 M sodium-potassium phosphate (30% ethylene glycol cryoprotectant), pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |