3T6F

Biotin complex of Y54F core streptavidin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-16
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97946
Spacegroup name	I 2 2 2
Unit cell lengths	46.611, 94.114, 104.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.919 - 1.220
R-factor	0.1294
Rwork	0.128
R-free	0.15060
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1mk5
RMSD bond length	0.011
RMSD bond angle	1.541
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.919	50.000	1.260
High resolution limit [Å]	1.220	2.630	1.220
Rmerge	0.121	0.100	0.613
Number of reflections	68448
<I/σ(I)>	23.065
Completeness [%]	99.8	99.8	98.4
Redundancy	13.4	13.8	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	60% saturated ammonium sulfate, 5% isopropanol (30% glycerol cryoprotectant), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K