3T6F
Biotin complex of Y54F core streptavidin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-16 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97946 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 46.611, 94.114, 104.595 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.919 - 1.220 |
R-factor | 0.1294 |
Rwork | 0.128 |
R-free | 0.15060 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1mk5 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.541 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.919 | 50.000 | 1.260 |
High resolution limit [Å] | 1.220 | 2.630 | 1.220 |
Rmerge | 0.121 | 0.100 | 0.613 |
Number of reflections | 68448 | ||
<I/σ(I)> | 23.065 | ||
Completeness [%] | 99.8 | 99.8 | 98.4 |
Redundancy | 13.4 | 13.8 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 60% saturated ammonium sulfate, 5% isopropanol (30% glycerol cryoprotectant), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |