3T6F
Biotin complex of Y54F core streptavidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-16 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.611, 94.114, 104.595 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.919 - 1.220 |
| R-factor | 0.1294 |
| Rwork | 0.128 |
| R-free | 0.15060 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mk5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.541 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.919 | 50.000 | 1.260 |
| High resolution limit [Å] | 1.220 | 2.630 | 1.220 |
| Rmerge | 0.121 | 0.100 | 0.613 |
| Number of reflections | 68448 | ||
| <I/σ(I)> | 23.065 | ||
| Completeness [%] | 99.8 | 99.8 | 98.4 |
| Redundancy | 13.4 | 13.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 60% saturated ammonium sulfate, 5% isopropanol (30% glycerol cryoprotectant), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
