3T5T
Vall from streptomyces hygroscopicus in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Collection date | 2011-03-21 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.671, 46.693, 123.879 |
| Unit cell angles | 90.00, 108.08, 90.00 |
Refinement procedure
| Resolution | 40.390 - 1.700 |
| R-factor | 0.18061 |
| Rwork | 0.179 |
| R-free | 0.20921 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t7d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.221 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.965 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 90507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
