3T57
Activity and Crystal Structure of Arabidopsis UDP-N-acetylglucosamine acyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00000 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 86.195, 86.195, 200.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.880 - 2.100 |
R-factor | 0.2085 |
Rwork | 0.207 |
R-free | 0.24075 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lxa |
RMSD bond length | 0.009 |
RMSD bond angle | 1.163 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.110 | ||
Number of reflections | 26374 | ||
<I/σ(I)> | 21.4 | ||
Completeness [%] | 99.6 | 96.4 | 100 |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.5 M ammonium sulfate, 0.1 M sodium citrate(pH 5.6), 1.0 M lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |