3T56
Crystal structure of the pre-extrusion state of the CusBA adaptor-transporter complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-23 |
| Wavelength(s) | 1.37 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 160.121, 160.121, 684.945 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.253 - 3.420 |
| R-factor | 0.261 |
| Rwork | 0.259 |
| R-free | 0.29440 |
| Structure solution method | MR-SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.747 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.540 |
| High resolution limit [Å] | 3.420 | 7.360 | 3.420 |
| Rmerge | 0.147 | 0.031 | 0.637 |
| Number of reflections | 46287 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.0 | 96.7 | 99.3 |
| Redundancy | 1.9 | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 10% PEG3350, 0.4M NH4SO4,10% Glycerol, pH 7.0, vapor diffusion, temperature 298K |
