3T4S

Arabidopsis histidine kinase 4 sensor domain in complex with kinetin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.99988
Spacegroup name	P 32 2 1
Unit cell lengths	59.860, 59.860, 297.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.590 - 1.600
R-factor	0.17879
Rwork	0.178
R-free	0.19952
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t4j
RMSD bond length	0.012
RMSD bond angle	1.415
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.590	1.640
High resolution limit [Å]	1.600	1.600
Number of reflections	83408
<I/σ(I)>	25.59	2.16
Completeness [%]	100.0	100
Redundancy	10.2	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	16% PEG 3,350, 0.2 M Na malonate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K