3T4K
Arabidopsis histidine kinase 4 sensor domain in complex with N-benzyladenine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.99988 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.140, 60.140, 297.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.110 - 1.770 |
R-factor | 0.18154 |
Rwork | 0.180 |
R-free | 0.20963 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t4j |
RMSD bond length | 0.014 |
RMSD bond angle | 1.386 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.110 | 1.820 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 62050 | |
<I/σ(I)> | 17.02 | 2.8 |
Completeness [%] | 99.4 | 91.6 |
Redundancy | 10.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 16% PEG 3,350, 0.2 M Na malonate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |