3T4J
Arabidopsis histidine kinase 4 sensor domain in complex with N-isopentenyl adenine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999880 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.870, 59.870, 297.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.800 - 1.650 |
| R-factor | 0.17683 |
| Rwork | 0.176 |
| R-free | 0.20077 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 76048 | |
| <I/σ(I)> | 30.64 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 19% PEG 3,350, 0.2 M Na malonate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
