3T47
Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2010-08-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97243 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.152, 94.356, 29.196 |
Unit cell angles | 90.00, 97.19, 90.00 |
Refinement procedure
Resolution | 28.967 - 1.301 |
R-factor | 0.1573 |
Rwork | 0.155 |
R-free | 0.18650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t48 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.082 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.350 |
High resolution limit [Å] | 1.300 | 2.800 | 1.300 |
Rmerge | 0.078 | 0.059 | 0.460 |
Number of reflections | 31662 | ||
<I/σ(I)> | 10.7 | ||
Completeness [%] | 92.2 | 96.3 | 89.3 |
Redundancy | 18.7 | 20.8 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 10mg/ml protein, 0.1M bis-Tris, 30% PEG 8000, 20 mM sodium thiocyanate, pH 5.0, vapor diffusion, hanging drop, temperature 293K |