3T43
Crystal Structure of HIV Epitope-Scaffold 4E10_1XIZA_S0_006_C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2007-04-17 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.060, 65.270, 73.100 |
Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
Resolution | 30.310 - 1.950 |
R-factor | 0.2063 |
Rwork | 0.204 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lf6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.040 |
Data reduction software | d*TREK (9.7D) |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.310 | 30.310 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.052 | 0.029 | 0.273 |
Rmeas | 0.052 | ||
Total number of observations | 74779 | 9230 | 4501 |
Number of reflections | 24452 | ||
<I/σ(I)> | 12.5 | 31.7 | 2 |
Completeness [%] | 99.7 | 99.4 | 99.6 |
Redundancy | 3.04 | 3.6 | 1.84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% PEG 4000, 20% Isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |