3T3T
1.38 A structure of human frataxin variant Q148G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Detector technology | CCD |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0971 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.410, 68.560, 89.970 |
Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
Resolution | 26.190 - 1.380 |
R-factor | 0.19947 |
Rwork | 0.198 |
R-free | 0.22656 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.032 |
RMSD bond angle | 2.500 |
Data reduction software | XFIT |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.190 | 1.416 |
High resolution limit [Å] | 1.380 | 1.380 |
Number of reflections | 111516 | |
Completeness [%] | 96.7 | 94.24 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 2.0 M ammonium sulfate, 0.1 M citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |