3T3N
Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 101.950, 116.780, 231.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.410 - 3.090 |
R-factor | 0.2066 |
Rwork | 0.202 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bk1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.090 |
Data reduction software | XDS (Package) |
Data scaling software | XDS (Package) |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 3.250 |
High resolution limit [Å] | 3.090 | 3.100 |
Rmerge | 0.100 | 0.641 |
<I/σ(I)> | 11.67 | 1.93 |
Completeness [%] | 94.6 | 85.1 |
Redundancy | 3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2-4% PEG 4K, 0.02M ammonium sulfate, 0.1M Na-MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |