3T3J
1.70 A structure of Friedreich's ataxia frataxin variant N146K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Detector technology | CCD |
| Collection date | 2010-11-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.470, 45.820, 38.900 |
| Unit cell angles | 90.00, 97.36, 90.00 |
Refinement procedure
| Resolution | 30.330 - 1.700 |
| R-factor | 0.21421 |
| Rwork | 0.214 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | XFIT |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.330 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 11654 | |
| Completeness [%] | 98.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 28% PEG2000 MME, 0.1 M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
