3T3E
Glycogen phosphorylase b in complex with GlcClU
Experimental procedure
| Experimental method | Chryssalis |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.234, 128.234, 116.354 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.910 - 2.150 |
| R-factor | 0.18007 |
| Rwork | 0.177 |
| R-free | 0.23267 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2gpn |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.940 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.097 | 0.525 |
| Number of reflections | 50243 | |
| <I/σ(I)> | 9.9 | 2.4 |
| Completeness [%] | 94.6 | 89.1 |
| Redundancy | 3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | T state crystals soaked with 2 mM solution of the inhibitor in the crystallization media for 2hrs, pH 6.7, SMALL TUBES, temperature 289K |
