3T3D
Glycogen phosphorylase b in complex with GlcU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2011-04-02 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.636, 128.636, 116.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.770 - 2.500 |
| R-factor | 0.15967 |
| Rwork | 0.156 |
| R-free | 0.22288 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2gpn |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.787 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.108 | 0.478 |
| Number of reflections | 33377 | |
| <I/σ(I)> | 17.6 | 2.5 |
| Completeness [%] | 97.7 | 99.2 |
| Redundancy | 8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | T state crystals soaked with 2 mM solution of the inhibitor in the crystallization media for 2hrs, pH 6.7, SMALL TUBES, temperature 289K |
