3T35

Arabidopsis thaliana dynamin-related protein 1A in postfission state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-20
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 61
Unit cell lengths	146.157, 146.157, 204.254
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.323 - 3.592
R-factor	0.2407
Rwork	0.238
R-free	0.29070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x2e
RMSD bond length	0.012
RMSD bond angle	1.735
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.592
Number of reflections	28872
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	160mM sodium formate, 16%(w/v) polyethylene glycol 3350, 40mM magnesium chloride, 6%(w/v) 2-Propanol, 20mM HEPES , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K