3T33
Crystal Structure of Arabidopsis GCR2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-29 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.849, 119.507, 77.239 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.050 - 2.250 |
R-factor | 0.1969 |
Rwork | 0.194 |
R-free | 0.25740 |
Structure solution method | SAD |
RMSD bond length | 0.021 |
RMSD bond angle | 1.783 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AutoSol |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 6.100 | 2.250 |
Rmerge | 0.070 | 0.031 | 0.432 |
Number of reflections | 21765 | ||
<I/σ(I)> | 10.9 | ||
Completeness [%] | 97.1 | 99.5 | 76.5 |
Redundancy | 13.8 | 13.4 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 1 M sodium acetate, 0.1 M ADA buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |