3T2M
Crystal Structure of NaK Channel N68D Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2009-04-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785736 |
| Spacegroup name | I 4 |
| Unit cell lengths | 68.088, 68.088, 89.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.044 - 1.953 |
| R-factor | 0.2039 |
| Rwork | 0.191 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.713 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 | 1.980 |
| High resolution limit [Å] | 1.950 | 2.150 | 1.950 |
| Number of reflections | 14516 | ||
| Completeness [%] | 100 | 84.5 | |
| Redundancy | 7.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 70% MPD, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
