3T22
Crystal structure of OxyR mutant from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.209, 64.356, 81.312 |
| Unit cell angles | 111.75, 90.56, 111.24 |
Refinement procedure
| Resolution | 18.358 - 2.200 |
| R-factor | 0.236 |
| Rwork | 0.234 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ho7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.205 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | RESOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.240 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.794 |
| Number of reflections | 69702 | |
| <I/σ(I)> | 5.8 | 0.9 |
| Completeness [%] | 90.9 | 88.8 |
| Redundancy | 3.53 | 3.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 30% PEG 3000, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
