3T1W
Structure of the four-domain fragment Fn7B89 of oncofetal fibronectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 211.980, 29.115, 82.241 |
Unit cell angles | 90.00, 111.42, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.23008 |
Rwork | 0.228 |
R-free | 0.27693 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fnf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 18943 | |
<I/σ(I)> | 18.8 | 3.5 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 26 %(w/v) PEG3350, 0.1 M Tris-HCl, 0.2 M NaCl, 5 %(v/v) Isopropanol, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |