3T1V
MglA bound to GDP in P2(1) tetrameric arrangement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97874 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.380, 48.870, 100.660 |
| Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
| Resolution | 48.800 - 2.400 |
| R-factor | 0.20052 |
| Rwork | 0.198 |
| R-free | 0.25621 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.773 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 34804 | |
| Completeness [%] | 98.4 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 65% MPD, 0.1M Tris-HCl , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
