3T1L
Crystal structure of human Galectin-3 in complex with methyl 2-O-acetyl-3-O-toluoyl-beta-D-talopyranoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2009-08-08 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.788, 58.125, 63.541 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.890 - 1.600 |
R-factor | 0.155 |
Rwork | 0.153 |
R-free | 0.18878 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.273 |
Data reduction software | SAINT |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.890 | 1.642 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 17607 | |
Completeness [%] | 94.2 | 75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 31% PEG 6000, 100mM MGCl2, 8mM beta merceptoethanol, 100mM Tris HCL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |