3T15

Structure of green-type Rubisco activase from tobacco

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0332
Spacegroup name	P 65
Unit cell lengths	103.635, 103.635, 56.675
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.950
R-factor	0.2292
Rwork	0.226
R-free	0.29620
Structure solution method	SIRAS
RMSD bond length	0.014
RMSD bond angle	1.449
Data reduction software	XDS
Data scaling software	SCALA (3.2.25)
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.848	56.725	3.110
High resolution limit [Å]	2.949	9.330	2.950
Rmerge	0.062	0.061	0.505
Total number of observations		1564	3960
Number of reflections	7453
<I/σ(I)>	14.6	8.6	1.5
Completeness [%]	99.9	98.6	100
Redundancy	4	6.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	50 mM MES-Na pH 6.0 and 350 mM magnesium formate, vapor diffusion, temperature 291K