3T0Z
Hsp90 N-terminal domain bound to ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 200 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.709, 46.780, 53.223 |
Unit cell angles | 90.00, 111.59, 90.00 |
Refinement procedure
Resolution | 49.488 - 2.192 |
R-factor | 0.2064 |
Rwork | 0.201 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yes |
RMSD bond length | 0.008 |
RMSD bond angle | 1.184 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.488 |
High resolution limit [Å] | 2.190 |
Rmerge | 0.165 |
Number of reflections | 11954 |
<I/σ(I)> | 21.118 |
Completeness [%] | 99.9 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 15~20% PEG 3350, 5mM magnesium chloride, 500mM ammonium phosphate, 100mM Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |