3T0Y

Structure of the PhyR anti-anti-sigma domain bound to the anti-sigma factor, NepR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	75.370, 105.320, 97.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.865 - 2.102
R-factor	0.2055
Rwork	0.200
R-free	0.25120
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.159
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100
Number of reflections	17325
Completeness [%]	95.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	287	100 mM MES (pH 6.0), 20% PEG 2000 MME, 200 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 287K