3T0W

Fluorogen activating protein M8VL in complex with dimethylindole red

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 2
Unit cell lengths	73.899, 83.533, 35.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.766 - 1.501
R-factor	0.1497
Rwork	0.148
R-free	0.18260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.296
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	35526
<I/σ(I)>	28.2	4.2
Completeness [%]	99.9	100
Redundancy	7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	35% PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 298K