3T0W
Fluorogen activating protein M8VL in complex with dimethylindole red
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 73.899, 83.533, 35.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.766 - 1.501 |
R-factor | 0.1497 |
Rwork | 0.148 |
R-free | 0.18260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.296 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.800 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 35526 | |
<I/σ(I)> | 28.2 | 4.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 35% PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 298K |