3T0Q
Motor Domain Structure of the Kar3-like kinesin from Ashbya gossypii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-07 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.866, 84.623, 101.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.640 - 2.350 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kar |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.283 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.077 | 0.244 |
| Number of reflections | 16073 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 95.6 | 97.8 |
| Redundancy | 3.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 14% PEG 3350, 125 mM sodium malonate, 1 mM TCEP and 0.1 M Bis-Tris propane pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
