3T0H
Structure insights into mechanisms of ATP hydrolysis and the activation of human Hsp90
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2010-12-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97931 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.287, 88.934, 99.672 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.179 - 1.200 |
R-factor | 0.151 |
Rwork | 0.150 |
R-free | 0.17080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xdl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.366 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.7_650) |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.483 |
High resolution limit [Å] | 1.200 |
Rmerge | 0.065 |
Number of reflections | 89825 |
<I/σ(I)> | 44.474 |
Completeness [%] | 99.0 |
Redundancy | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20-25% (w/v) PEG 2000 monomethyl ether, 200mM magnesium chloride, 100mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |