3SZX

Crystal Structure of the Triplet Repeat in Myotonic Dystrophy Reveals Heterogeneous 1x1 Nucleotide UU Internal Loop Conformations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97855
Spacegroup name	H 3
Unit cell lengths	39.505, 39.505, 155.887
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.410 - 2.204
R-factor	0.2274
Rwork	0.222
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	2.150
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.410	4.740	2.280
High resolution limit [Å]	2.200	3.760	2.200
Number of reflections	4571
Completeness [%]	99.8	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	10 mM magnesium sulfate, 50 mM sodium cacodylate, 1.8 M lithium sulfate , pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K