3SZP
Full-length structure of the Vibrio cholerae virulence activator, AphB, a member of the LTTR protein family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 100.209, 106.100, 167.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.850 - 2.202 |
| R-factor | 0.2093 |
| Rwork | 0.208 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEMET DERIVATIVE SOLVED BY SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (AutoMR) |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 45546 | |
| <I/σ(I)> | 13.12 | 4.1 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 4 M sodium formate, 0.01 M betaine monohydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
