3SZI
Structure of apo shwanavidin (P21 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.518, 110.564, 84.200 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 83.920 - 1.400 |
| R-factor | 0.15916 |
| Rwork | 0.157 |
| R-free | 0.19205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 83.920 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 236160 |
| Completeness [%] | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micobatch | 293 | 170 mM Bis-Tris pH 6.0-6.8, 2.0 M Sodium Formate, micobatch, temperature 293K |
