3SYX
Crystal Structure of the WH1 domain from human sprouty-related, EVH1 domain-containing protein. Northeast Structural Genomics Consortium Target HR5538B.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97896 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 52.754, 52.754, 121.638 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.377 - 2.453 |
R-factor | 0.23 |
Rwork | 0.228 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xod |
RMSD bond length | 0.009 |
RMSD bond angle | 1.664 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.057 | 0.691 |
Number of reflections | 27739 | |
<I/σ(I)> | 29.6 | 2.5 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 4.9 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch crystallization under oil | 6 | 277 | 12% PEG 20000, 0.1 M potassium acetate, 10 mM yttrium(III) chloride, 0.1 M MES, pH 6.0, Microbatch crystallization under oil, temperature 277K |