3SYW
Crystal Structure of the Triplet Repeat in Myotonic Dystrophy Reveals Heterogeneous 1x1 Nucleotide UU Internal Loop Conformations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-09 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 46.535, 46.535, 135.001 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.600 - 1.570 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.600 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.057 | 0.487 |
| Number of reflections | 16712 | |
| <I/σ(I)> | 48.99 | 3.1 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 291 | 15 mM magnesium acetate, 50 mM sodium cacodylate, pH 6.0, 1.7 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
