3SYV
Crystal structure of mPACSIN 3 F-BAR domain mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.570, 108.901, 222.319 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 48.900 - 3.100 |
| R-factor | 0.28345 |
| Rwork | 0.281 |
| R-free | 0.33683 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.038 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 99512 | |
| Completeness [%] | 98.7 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 0.5M Ammonium sulfate, 0.1M Sodium citrate tribasic dihydrate pH 5.6, 1.0M Lithium sulfate monohydrate, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
