3SYO

Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with sodium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 4 21 2
Unit cell lengths	85.737, 85.737, 178.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.680 - 3.540
R-factor	0.2613
Rwork	0.260
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e4f
RMSD bond length	0.008
RMSD bond angle	1.118
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.685	41.685	3.700
High resolution limit [Å]	3.540	7.680	3.570
Rmerge	0.158	0.058	0.729
Number of reflections	7975
<I/σ(I)>	6.4
Completeness [%]	92.0	90.2	76.8
Redundancy	8.1	7.9	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	50 mM sodium citrate, pH 6.0, 1 M sodium chloride, 30-35% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K