3SYJ
Crystal structure of the Haemophilus influenzae Hap adhesin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.410, 137.200, 209.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.600 - 2.200 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3H09 and 2WXR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.918 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.600 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 60596 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 97.6 | 89 |
| Redundancy | 4.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 100mM sodium citrate, 14% (w/v) PEG 4000, 100mM ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
