Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.600, 80.900, 130.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.773 - 1.489 |
R-factor | 0.1895 |
Rwork | 0.187 |
R-free | 0.21210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | STARTING MODEL: PDB ENTRY 1Q9O S45-18 FAB UNLIGANDED |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.770 |
High resolution limit [Å] | 1.490 |
Number of reflections | 73625 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | KDO trisaccharide, magnesium chloride, zinc chloride, ethylene glycol, glycerol, PEG 4000, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |