3SXU
Structure of the E. coli SSB-DNA polymerase III interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.12718 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 79.927, 79.927, 155.045 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 63.210 - 1.850 |
R-factor | 0.18564 |
Rwork | 0.184 |
R-free | 0.20846 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1em8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.409 | |
Number of reflections | 25843 | |
<I/σ(I)> | 63.02 | 10.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.2 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 25% PEG 4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |