3SXU
Structure of the E. coli SSB-DNA polymerase III interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12718 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 79.927, 79.927, 155.045 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.210 - 1.850 |
| R-factor | 0.18564 |
| Rwork | 0.184 |
| R-free | 0.20846 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1em8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.409 | |
| Number of reflections | 25843 | |
| <I/σ(I)> | 63.02 | 10.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.2 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 25% PEG 4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
