3SXL
SEX-LETHAL RNA RECOGNITION DOMAINS 1 AND 2 FROM DROSOPHILA MELANOGASTER
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Wavelength(s) | 1.06879, 0.98001, 0.97957, 1.2000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.910, 124.470, 50.930 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.218 * |
| Rwork | 0.221 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.000 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.069 * | |
| Number of reflections | 16847 | |
| <I/σ(I)> | 13.4 | 5.7 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | glycerol | 10 (%) | |
| 3 | 1 | drop | HEPES | 5 (mM) | |
| 4 | 1 | reservoir | PEG2000 MME | 20 (%) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
