3SXL

SEX-LETHAL RNA RECOGNITION DOMAINS 1 AND 2 FROM DROSOPHILA MELANOGASTER

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	120
Detector technology	CCD
Wavelength(s)	1.06879, 0.98001, 0.97957, 1.2000
Spacegroup name	P 1 21 1
Unit cell lengths	50.910, 124.470, 50.930
Unit cell angles	90.00, 120.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.218 *
Rwork	0.221
R-free	0.26400
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	2.000
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700	2.700
Rmerge	0.069 *
Number of reflections	16847
<I/σ(I)>	13.4	5.7
Completeness [%]	99.8
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18 (mg/ml)
2	1	drop	glycerol	10 (%)
3	1	drop	HEPES	5 (mM)
4	1	reservoir	PEG2000 MME	20 (%)
5	1	reservoir	HEPES	0.1 (M)
6	1	reservoir	ammonium sulfate	0.2 (M)