3SWP

ANAC019 NAC domain in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-16
Detector	MAR CCD 165 mm
Wavelength(s)	1.038
Spacegroup name	P 21 21 21
Unit cell lengths	69.140, 105.470, 175.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.966 - 4.113
R-factor	0.2688
Rwork	0.260
R-free	0.34900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1UT7 + B-DNA model
RMSD bond length	0.015
RMSD bond angle	2.484
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.966	4.400
High resolution limit [Å]	4.113	4.113
Number of reflections	10348
Completeness [%]	99.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.01 M magnesium sulfate, 0.05 M MES, pH 6.5, 0.5% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K