3SWL
Crystal Structure Analysis of H74A Mutant of Human CLIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-10-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.199, 41.542, 66.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.800 - 2.350 |
| R-factor | 0.2473 |
| Rwork | 0.242 |
| R-free | 0.33780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.574 |
| Data reduction software | SAINT (V7.34A) |
| Data scaling software | SAINT (V7.34A) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 1.957 | ||
| High resolution limit [Å] | 2.350 | 4.071 | 1.890 |
| <I/σ(I)> | 196.431 | ||
| Completeness [%] | 98.8 | ||
| Redundancy | 9.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris, 30% (v/v) PEG 550 monomethyl ether, 0.05 M calcium chloride, 2.5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
