3SVU
Crystal structure of mKate mutant S143C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 140 |
| Detector technology | CCD |
| Collection date | 2010-09-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 41 |
| Unit cell lengths | 161.621, 161.621, 76.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.049 - 2.695 |
| R-factor | 0.1955 |
| Rwork | 0.189 |
| R-free | 0.26950 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.228 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 26725 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298.15 | 0.1M tris-HCl, 0.1M ammonium citrate dibasic , 18% PEG3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
