3SVI
Structure of the Pto-binding domain of HopPmaL generated by limited thermolysin digestion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.277, 57.277, 55.658 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.615 - 1.800 |
| R-factor | 0.1874 |
| Rwork | 0.186 |
| R-free | 0.21780 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.615 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.071 | 0.428 |
| Number of reflections | 9824 | |
| <I/σ(I)> | 43.429 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.2 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1M Hepes ph7.5, 0.1M NaCl, 1.6M Ammonium Sulphate, 0.2 mg/ml thermolysin was added to the protein mixture. Paratone-N oil was used for cryoprotection, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
