3SVE
Engineered low-affinity halide-binding protein derived from YFP: bromide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.91993 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.296, 62.212, 69.549 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.775 - 1.492 |
R-factor | 0.1543 |
Rwork | 0.153 |
R-free | 0.17870 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3sst |
RMSD bond length | 0.019 |
RMSD bond angle | 1.820 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.282 | 50.000 | 1.530 |
High resolution limit [Å] | 1.492 | 4.070 | 1.500 |
Rmerge | 0.077 | 0.061 | 0.468 |
Number of reflections | 36416 | ||
<I/σ(I)> | 13.8 | ||
Completeness [%] | 98.8 | 99.8 | 90.3 |
Redundancy | 6.9 | 6.3 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | 18% PEG2000, 50 mM sodium acetate, 90 mM magnesium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |