3SVC
Engineered medium-affinity halide-binding protein derived from YFP: chloride complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1169 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.615, 62.664, 65.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.840 - 1.310 |
R-factor | 0.1356 |
Rwork | 0.134 |
R-free | 0.16430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sst |
RMSD bond length | 0.009 |
RMSD bond angle | 1.307 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.330 |
High resolution limit [Å] | 1.310 | 3.560 | 1.310 |
Rmerge | 0.049 | 0.033 | 0.486 |
Number of reflections | 51271 | ||
<I/σ(I)> | 16 | ||
Completeness [%] | 98.3 | 85.9 | 97.8 |
Redundancy | 6.7 | 6.2 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 20% PEG2000, 50 mM sodium acetate, 90 mM magnesium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |