3SV0
Crystal structure of casein kinase-1 like protein in plant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-05-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.828, 69.604, 55.849 |
Unit cell angles | 90.00, 109.47, 90.00 |
Refinement procedure
Resolution | 30.830 - 2.000 |
R-factor | 0.19945 |
Rwork | 0.197 |
R-free | 0.25019 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.059 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 26484 | |
Completeness [%] | 30.0 | 90 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 298 | 0.1M HEPES sodium, 5%(v/v) 2-Propanol, 10%(w/v) Polyethylene glycol 4000, pH 7.5, EVAPORATION, temperature 298K |