3SUI
Crystal structure of ca2+-calmodulin in complex with a trpv1 c-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 41.681, 41.681, 341.533 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.100 - 1.950 |
| R-factor | 0.19437 |
| Rwork | 0.192 |
| R-free | 0.24837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dve |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.519 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.052 | 0.469 |
| Number of reflections | 13862 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 98.0 | 91.2 |
| Redundancy | 5.4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M MES PH6.0, 2.2-2.8M AMMONIUM SULFATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
